Antibacterial Activity of Clove-derived Endophytic Bacteria of Niallia nealsonii DCL1 and Bacillus paranthracis DCL3
DOI:
https://doi.org/10.25134/quagga.v18i1.470Keywords:
Antibacterial, disc diffusion, endophytic bacteria, pathogenic bacteria, cloveAbstract
Pathogenic bacterial infection is still a major health problem that causes death. Endophytic bacteria in medicinal plants have the potential as antibacterial agents. Thus, endophytic bacteria in the medicinal plant, clove (Syzygium aromaticum), allow it to produce the same bioactive compounds as those contained in its host. This study aimed to analyze the antibacterial activity of five endophytic bacterial isolates from clove leaves. Antibacterial activity was evaluated using disc diffusion assay. Based on the results, isolate Niallia nealsonii DCL1 have strong antibacterial activity against Escherichia coli ATCC 8739, while Bacillus paranthracis DCL3 isolate has intermediate antibacterial activity against Pseudomonas aeruginosa ATCC 15442. In addition, extracellular metabolites of these two isolates of DCL1 and DCL3 which harvested at stationary phase showed antibacterial activity against and E. coli ATCC 8739 and P. aeruginosa ATCC 15442. These data indicate that the potential antibacterial compounds from DCL1 and DCL3 are considered extracellular secondary metabolites. Further analysis on the mode of antibacterial action should be carried out for further medical application.
References
Afrendi, E., Prastya, M.E., Astuti, R.I., Wahyuni, W.T., Batubara, I., 2023. Bioactivity of the Ethanol Extract of Clove (Syzygium aromaticum) as Antitoxin. Int J Food Sci 2023. https://doi.org/10.1155/2023/3245210
Almulhim, A.S., Alotaibi, F.M., 2018. Comparison of broad-spectrum antibiotics and narrow-spectrum antibiotics in the treatment of lower extremity cellulitis. Int J Health Sci (Qassim) 12, 3.
Amini, S., Astuti, R.I., Batubara, I., 2022. Apoptosis in Yeast is Modulated by Clove Leaf Extract and Eugenol Potentially by Interfering Caspase YCA1. Online J Biol Sci 22, 388–394. https://doi.org/10.3844/ojbsci.2022.388.394
Anand, U., Jacobo-Herrera, N., Altemimi, A., Lakhssassi, N., 2019. A Comprehensive Review on Medicinal Plants as Antimicrobial Therapeutics: Potential Avenues of Biocompatible Drug Discovery. Metabolites 9. https://doi.org/10.3390/METABO9110258
Asfahani, N.F., Astuti, R.I., Listiyowati, S., Batubara, I., 2023. Quercetin-Containing Extract from Clove Syzygium aromaticum L. Endophytic Bacteria, Fictibacillus phosphorivorans P1U2, Exhibits Antimutagenic Activity in Yeast Saccharomyces cerevisiae. Hayati 30, 734–742. https://doi.org/10.4308/hjb.30.4.734-742
Barboza, J.N., da Silva Maia Bezerra Filho, C., Silva, R.O., Medeiros, J.V.R., de Sousa, D.P., 2018. An overview on the anti-inflammatory potential and antioxidant profile of eugenol. Oxid Med Cell Longev 2018. https://doi.org/10.1155/2018/3957262
Cortés-Rojas, D.F., de Souza, C.R.F., Oliveira, W.P., 2014. Clove (Syzygium aromaticum): A precious spice. Asian Pac J Trop Biomed 4, 90–96. https://doi.org/10.1016/S2221-1691(14)60215-X
Dany, R.R., Astuti, R.I., Priyanto, J.A., 2023. The clove essential oil and its combination with galangal oil exhibit antibacterial activity, potential as antiseptic agent. IOP Conf Ser Earth Environ Sci 1271, 012076. https://doi.org/10.1088/1755-1315/1271/1/012076
Gerstein, A.C., Rosenberg, A., Hecht, I., Berman, J., 2016. DiskimageR: Quantification of resistance and tolerance to antimicrobial drugs using disk diffusion assays. Microbiology (United Kingdom) 162, 1059–1068. https://doi.org/10.1099/mic.0.000295
Jubeh, B., Breijyeh, Z., Karaman, R., 2020. Resistance of Gram-Positive Bacteria to Current Antibacterial Agents and Overcoming Approaches. Molecules 25, 2888. https://doi.org/10.3390/MOLECULES25122888
Kapoor, G., Saigal, S., Elongavan, A., 2017. Action and resistance mechanisms of antibiotics: A guide for clinicians. J Anaesthesiol Clin Pharmacol 33, 300. https://doi.org/10.4103/JOACP.JOACP_349_15
Larsson, D.G.J., Flach, C.F., 2021. Antibiotic resistance in the environment. Nature Reviews Microbiology 2021 20:5 20, 257–269. https://doi.org/10.1038/s41579-021-00649-x
Nandhini, P., Kumar, P., Mickymaray, S., Alothaim, A.S., Somasundaram, J., Rajan, M., 2022. Recent Developments in Methicillin-Resistant Staphylococcus aureus (MRSA) Treatment: A Review. Antibiotics 11. https://doi.org/10.3390/ANTIBIOTICS11050606
Priyanto, J.A., Prastya, M.E., Astuti, R.I., Kristiana, R., 2023a. The Antibacterial and Antibiofilm Activities of the Endophytic Bacteria Associated with Archidendron pauciflorum against Multidrug-Resistant Strains. Appl Biochem Biotechnol 195, 6653–6674. https://doi.org/10.1007/S12010-023-04382-4/METRICS
Priyanto, J.A., Prastya, M.E., Astuti, R.I., Kristiana, R., 2023b. The Antibacterial and Antibiofilm Activities of the Endophytic Bacteria Associated with Archidendron pauciflorum against Multidrug-Resistant Strains. Appl Biochem Biotechnol 195, 6653–6674. https://doi.org/10.1007/S12010-023-04382-4
Raheem, N., Straus, S.K., 2019. Mechanisms of Action for Antimicrobial Peptides With Antibacterial and Antibiofilm Functions. Front Microbiol 10, 501450. https://doi.org/10.3389/FMICB.2019.02866/BIBTEX
Rahmi, D., Yunilawati, R., Jati, B.N., Setiawati, I., Riyanto, A., Batubara, I., Astuti, R.I., 2021. Antiaging and Skin Irritation Potential of Four Main Indonesian Essential Oils. Cosmetics 2021, Vol. 8, Page 94 8, 94. https://doi.org/10.3390/COSMETICS8040094
Rakhman, I.M.N., Priyanto, J.A., Astuti, R.I., 2024. Aktivitas Antimikrob Minyak Atsiri dan Potensinya sebagai Antiseptik : Jurnal Sumberdaya Hayati 10, 41–47. https://doi.org/10.29244/JSDH.10.2.41-47
Singh, M., Kumar, A., Singh, R., Pandey, K.D., 2017. Endophytic bacteria: a new source of bioactive compounds. 3 Biotech 7. https://doi.org/10.1007/S13205-017-0942-Z
Utami, L.A., Wahyuni, W.T., Mubarik, N.R., Astuti, R.I., 2023. Endophytic bacteria of clove (Syzygium aromaticum L.) leaves produce metabolites with antioxidant and anti-aging properties. J Appl Pharm Sci 13, 241–250. https://doi.org/10.7324/JAPS.2023.93258
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